IR Home
DAO
Home
Editors
Forthcoming
Information
Journals
Home
MEPS
AME
CR
DAO
ESEP
ESR
Search
Subscribe
Book Series
EE Books
Top Books
ESEP Books
Order
EEIU Brochures
(pdf format)
Discussion Forums
Home
Research
IR Research
Institutions
International Ecology Institute
Eco-Ethics International Union
Foundation
Otto Kinne Foundation
 | |
DAO 60:41-48 (2004)
|
Abstract
|
Some immune parameters in carp Cyprinus carpio susceptible and resistant to the haemoflagellate Trypanoplasma borreli
J. P. Scharsack1, D. Steinhagen1,*, W. Körting1, B. Wagner2, W. Leibold2, H. J. Schuberth2
1Fish Disease Research Unit, and
2Immunology Unit, School of Veterinary Medicine, Hannover, PO Box 711180, 30545 Hannover, Germany
*Corresponding author. Email: dieter.steinhagen@tiho-hannover.de
ABSTRACT: The present study addresses aspects of the (specific) immune response of carp to the haemoflagellate Trypanoplasma borreli. Sera of resistant carp contained antibodies, which agglutinated the flagellates in vitro. When flagellates
were incubated in fish sera from resistant carp, binding of antibodies to flagellates could be demonstrated by flow cytometry, and T. borreli were effectively killed. Heat-treatment of the sera prevented killing, indicating that complement
activation is important for the control of a T. borreli infection. Sera of carp that were highly susceptible to infection with T. borreli contained no antibodies capable of binding to or killing the parasite. Furthermore, specific antibodies
were not generated after experimental infection. This lack of antibody production in susceptible carp is not due to a general unresponsiveness of lymphoid cells, since peripheral blood leukocytes (PBL) from susceptible and resistant carp responded to
mitogenic stimuli in vitro with lymphocyte proliferation in a similar manner. However, viable flagellates were significantly less able to stimulate proliferation of PBL from susceptible carp. In vitro-produced culture supernatants of freshly
isolated PBL from both carp lines (but not those of head kidney cells) differentially modulated the mitogen-induced proliferation of PBL from susceptible and resistant carp. The supernatants enhanced the proliferation of leukocytes obtained from
individuals from the same carp line, but suppressed the mitogen-induced proliferation of PBL from the other line tested. This indicates that lymphoid cells from susceptible and resistant carp differ in their spectrum of spontaneously produced
immunomodulatory mediators. Whether this is decisive for a T. borreli-specific and successful immune response is discussed.
KEY WORDS: Trypanoplasma borreli · Disease susceptibility · Leukocyte activation · Immune modulation · Specific immune responses
Full text in pdf format
Published in DAO Vol.
60, No. 1
(2004) on July 5
Print ISSN: 0177-5103; Online ISSN: 1616-1580.
Copyright © Inter-Research, Oldendorf/Luhe, 2004
|
