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White spot syndrome virus (WSSV) infectivity for Artemia at different developmental stagesQin Li1,*, Jinghai Zhang1, Yingjie Chen1, Feng Yang21Department of Biochemistry, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, PR China 2Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, SOA, 184 Daxue Road, Xiamen 361005, PR ChinaABSTRACT: White spot syndrome virus (WSSV) is a major pathogen of cultivated shrimp, but its host range includes a large number of crustaceans. In this investigation, Artemia franciscana was tested for susceptibility to WSSV by the oral route. Both instars and adults were challenged, and the presence of WSSV was followed through to reproductive cysts and offspring using PCR. WSSV caused a much lower cumulative mortality in Artemia than in cultivated shrimp by 10 d post-challenge. Instars, adults and reproductive cysts were PCR positive. However, the virus was undetectable by PCR in nauplii that had hatched from PCR-positive reproductive cysts. The data indicate that WSSV or WSSV genomic DNA can be vertically transmitted from WSSV-PCR-positive instars to reproductive cysts, but this DNA is removed during hatching.
KEY WORDS: Susceptibility · Artemia · WSSV · Infection · PCR
Published in DAO Vol.
57, No. 3
(2003) on December 29
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