IR Home
DAO
Home
Editors
Forthcoming
Information
Journals
Home
MEPS
AME
CR
DAO
ESEP
Search
Subscribe
Book Series
EE Books
Top Books
ESEP Books
Order
EEIU Brochures
(pdf format)
Discussion Forums
Home
Research
Endangered Species Programs
Institutions
International Ecology Institute
Eco-Ethics International Union
Foundation
Otto Kinne Foundation
 | |
DAO 57:231-235 (2003)
|
Abstract
|
Genetic and morphologic differentiation of Bolbophorus confusus and B. levantinus (Digenea: Diplostomatidae), based on rDNA SSU polymorphism and SEM
R. Dzikowski1,3,*, M. G. Levy2, M. F. Poore2, J. R. Flowers2, I. Paperna1
1Department of Animal Sciences, Faculty of Agriculture, Food and Environmental Sciences, the Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
2Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough St., Raleigh 27606, North Carolina, USA
3Present address: Department of Microbiology and Immunology, Weill Medical College of Cornwell University, 1300 York Avenue, W-704, Box 62, New York, New York 10021, USA
*Email: rrd2001@med.cornell.edu
ABSTRACT: Metacercariae of Bolbophorus species are serious pathogens of farmed fish. Molecular diagnostic tools, capable of identifying and differentiating these parasites, may assist in the development of rationale control strategies. The rDNA 18S
(small sub-unit: SSU) genes of adult B. confusus and B. levantinus obtained from a pelican, Pelecanus onocrotalus, and a night heron, Nycticorax nycticorax, respectively, were amplified, sequenced, and aligned. Based on this
alignment, we developed a genetic differentiation assay between B. confusus and B. levantinus. These 2 species were compared genetically with the North American species B. damnificus and Bolbophorus sp. ('Type 2'). The
relationship between species is outlined and discussed. In addition to the molecular study, specimens of B. confusus and B. levantinus were compared morphologically, using scanning electron microscopy. Morphologic analysis revealed
interspecific differences in details of the holdfast organ and the position of the acetabulum.
KEY WORDS: Digenea · Diplostomatidae · Bolbophorus confusus · Bolobphorus levantinus · 18S (SSU) rDNA gene · SEM
Full text in pdf format
Published in DAO Vol.
57, No. 3
(2003) on December 29
Print ISSN: 0177-5103; Online ISSN: 1616-1580.
Copyright © Inter-Research, Oldendorf/Luhe, 2003
|
