IR Home
DAO
Home
Editors
Forthcoming
Information
Journals
Home
MEPS
AME
CR
DAO
ESEP
Search
Subscribe
Book Series
EE Books
Top Books
ESEP Books
Order
EEIU Brochures
(pdf format)
Discussion Forums
Home
Research
Endangered Species Programs
Institutions
International Ecology Institute
Eco-Ethics International Union
Foundation
Otto Kinne Foundation
 | |
DAO 57:67-75 (2003)
|
Abstract
|
Minor effect of depletion of resident macrophages from peritoneal cavity on resistance of common carp Cyprinus carpio to blood flagellates
Jeroen P. J. Saeij1, Adrie Groeneveld1, Nico Van Rooijen2, Olga L. M. Haenen3, Geert F. Wiegertjes1,*
1Cell Biology and Immunology Group, Wageningen Institute of Animal Sciences, Wageningen University, PO Box 338, 6700 AH Wageningen, The Netherlands
2Free University, Department of Molecular Cell Biology, Faculty of Medicine, POB 7057, 1007 MB Amsterdam, The Netherlands
3Central Institute for Animal Disease Control, POB 2004, 8203 AA Lelystad, The Netherlands
*Corresponding author. Email: geert.wiegertjes@wur.nl
ABSTRACT: Carp Cyprinus carpio macrophages were depleted by intraperitoneal (i.p.) injection of clodronate-liposomes for the in vivo study of the effect of macrophage depletion on the resistance of carp to infection with blood flagellate
parasites. Clodronate released inside the cell induces apoptosis of (murine) macrophages. Following i.p. injection of carp with liposomes alone, but not with Trypanoplasma borreli, neutrophilic granulocytes rapidly migrated from the head kidney to
the peritoneal cavity. The majority of liposomes in the peritoneal cavity were not taken up by newly arrived neutrophilic granulocytes, however, but by resident macrophages. After 2 i.p. injections of clodronate-liposomes, the percentage of macrophages
present in the peritoneal cavity was significantly reduced, as evaluated by flow cytometry. Macrophage-depleted carp that were infected i.p. with T. borreli suffered from high mortality. However, these fish did not show lethal parasitaemia but did
show clear bacteraemia. Macrophage-depleted carp that were infected i.p. with Trypanosoma carassii showed a minor increase in parasitaemia. In addition, macrophage-depleted carp, immune to T. borreli as a result of having survived a prior
infection, remained immune to i.p. reinfection with T. borreli. Succesful depletion of peritoneal macrophages seemed to have a minor effect on the resistance of carp against blood flagellates. However, carp macrophages are essential as a first line
of defence against (bacterial) infection.
KEY WORDS: Cyprinus carpio · Macrophages · Clodronate · Trypanoplasma borreli · Trypanosoma carassii
Full text in pdf format
Published in DAO Vol.
57, No. 1-2
(2003) on December 3
Print ISSN: 0177-5103; Online ISSN: 1616-1580.
Copyright © Inter-Research, Oldendorf/Luhe, 2003
|
