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DAO 57:141-146 (2003)
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Abstract
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Detection by PCR of hepatopancreatic parvovirus (HPV) and other viruses in hatchery-reared Penaeus monodon postlarvae
Rudrappa K. Umesha1, Arumugam Uma2, Subhendu K. Otta1, Indrani Karunasagar1,*, Iddya Karunasagar1
1Department of Fishery Microbiology, University of Agricultural Sciences, College of Fisheries, Mangalore 575 002, India
2Shrimp Disease Diagnosis Laboratory, Tamilnadu Veterinary and Animal Sciences University, Madhavaram Milk
Colony, Chennai-600051, India
*Corresponding author. Email: mircen@sancharnet.in
ABSTRACT: The prevalence of hepatopancreatic parvovirus (HPV), monodon baculovirus (MBV) and white spot syndrome virus (WSSV) in samples of Penaeus monodon postlarvae (PL10 to PL20, 10 to 20 d old postlarvae) in India was studied by PCR. Samples
collected from different hatcheries, and also samples submitted by farmers from different coastal states, were analyzed. HPV was detected in 34% of the hatchery samples and 31% of the samples submitted by farmers, using a primer set designed for detection
of HPV from P. monodon in Thailand. However, none of these samples were positive using primers designed for detection of HPV from P. chinensis in Korea. This indicated that HPV from India was more closely related to HPV from P.
monodon in Thailand. MBV was detected in 64% of the samples submitted by the farmers and 71% of the hatchery samples. A total of 84% of the samples submitted by farmers, and 91% of the hatchery samples, were found positive for WSSV. Prevalence of
concurrent infections by HPV, MBV and WSSV was 27% in hatchery samples and 29% in samples submitted by farmers. Only 8% of the hatchery samples and 16% of the samples submitted by farmers were negative for all 3 viruses. This is the first report on the
prevalence of HPV in P. monodon postlarvae from India.
KEY WORDS: HPV · MBV · WSSV · PCR · Penaeus monodon · Postlarvae · HPVmon
Full text in pdf format
Published in DAO Vol.
57, No. 1-2
(2003) on December 3
Print ISSN: 0177-5103; Online ISSN: 1616-1580.
Copyright © Inter-Research, Oldendorf/Luhe, 2003
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