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DAO 56:59-64 (2003)
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Abstract
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Cryo-archiving of Batrachochytrium dendrobatidis and other chytridiomycetes
D. G. Boyle1, A. D. Hyatt1,*, P. Daszak2, L. Berger1, J. E. Longcore3, David Porter3, S. G. Hengstberger1, V. Olsen1
1Australian Animal Health Laboratory, CSIRO Livestock Industries, Private Bag 24, Geelong, Victoria 3220, Australia
2Consortium for Conservation Medicine, 61 Route 9W, Palisades, New York 10964-8000, USA
3Department of Biological Sciences, University of Maine, Orono, Maine 04469-5722, USA
*Corresponding author. Email: alex.hyatt@csiro.au
ABSTRACT: Batrachochytrium dendrobatidis is a major pathogen of frogs worldwide. It has been associated with catastrophic declines of frog populations including those in pristine habitats in Queensland, Australia. To facilitate genetic and disease
studies of this fungus and related species, it is essential to have a reliable long-term storage method to maintain genetic integrity of isolates. We have adapted well-established techniques used for the long-term storage of tissue-culture cell lines to
the preservation of B. dendrobatidis and other chytridiomycetes. This simple method has allowed us to recover these fungi from storage at -80°C and in liquid nitrogen over an extended period. With this technique it is now possible to preserve
saprobic and parasitic isolates from a variety of environmental and disease situations for comparative genetic and biological studies.
KEY WORDS: Chytrids · Batrachochytrium dendrobatidis · Storage · Cryopreservation · Amphibian declines
Full text in pdf format
Published in DAO Vol.
56, No. 1
(2003) on August 15
Print ISSN: 0177-5103; Online ISSN: 1616-1580.
Copyright © Inter-Research, Oldendorf/Luhe, 2003
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