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DAO 55:11-16 (2003)
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Abstract
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Molecular characterisation of Neoparamoeba strains isolated from gills of Scophthalmus maximus
Ivan Fiala1,2, Iva Dyková1,2,*
1Institute of Parasitology, Academy of Sciences of the Czech Republic and
2Faculty of Biological Sciences, University of South Bohemia, Brani$ovská 31, 370 05 >eské Bud[caron]ejovice, Czech Republic
*Corresponding author. Email: iva@paru.cas.cz
ABSTRACT: Small subunit ribosomal RNA gene sequences were determined for 5 amoeba strains of the genus Neoparamoeba Page, 1987 that were isolated from gills of Scophthalmus maximus (Linnaeus, 1758). Phylogenetic analyses revealed that 2 of 5
morphologically indistinguishable strains clustered with 6 strains identified previously as N. pemaquidensis (Page, 1970). Three strains branched as a clade separated from N. pemaquidenis and N. aestuarina (Page,
1970) clades. Our analyses suggest that these 3 strains could be representatives of an independent species. In a more comprehensive eukaryotic tree, strains belonging to Neoparamoeba spp. formed a monophyletic group with a sister-group
relationship to Vannella anglica Page, 1980. They did not cluster with Gymnamoebae of the families Hartmannellidae, Flabellulidae, Leptomyxidae or Amoebidae presently available in GenBank.
KEY WORDS: Paramoeba · Neoparamoeba · SSU rDNA · Phylogenetic position
Full text in pdf format
Published in DAO Vol.
55, No. 1
(2003) on June 20
Print ISSN: 0177-5103; Online ISSN: 1616-1580.
Copyright © Inter-Research, Oldendorf/Luhe, 2003
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