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Detection of Myxobolus rotundus (Myxozoa: Myxosporea) in skin mucus of crucian carp Carassius auratus auratus using a monoclonal antibodyY. S. Lu, P. Nie*, B. J. SunState Key Laboratory of Freshwater Ecology and Biotechnology and Laboratory of Fish Diseases, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, Hubei Province, PR ChinaABSTRACT: Diagnosis of myxosporean Myxobolus rotundus infection was conducted by examining skin mucus from the infected crucian carp Carassius auratus auratus with a monoclonal antibody, MAb 2D12, raised previously against the parasite. A positive reaction was observed in skin mucus collected from infected fish, and spores and pre-spore stages of the parasite were identified by the MAb 2D12. It was also demonstrated that M. rotundus infection can be successfully detected by a simple method, enzyme-linked immunosorbent assay (ELISA), and that skin mucus collected from infected fish skin had a significantly higher optical density (OD) value than that from uninfected fish.
KEY WORDS: Myxosporean · Myxobolus rotundus · Crucian carp · Carassius auratus auratus · Skin mucus · Monoclonal antibody · Diagnosis
Published in DAO Vol.
54, No. 2
(2003) on March 31
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