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DAO 49:139-144 (2002)
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Abstract
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PCR-based assays for the fish pathogen Aeromonas salmonicida. II. Further evaluation and validation of three PCR primer sets with infected fish
Helen K. Byers1, Rocco C. Cipriano2, Nicholas Gudkovs1, Mark St. J. Crane1,*
1Australian Animal Health Laboratory, CSIRO Livestock Industries, Private Bag 24, Geelong VIC 3220 Australia
2National Fish Health Research Laboratory, United States Geological Survey, Biological Resources Division, 1700 Leetown Road, Kearneysville, West Virginia 25430, USA
*Corresponding author. E-mail: mark.crane@csiro.au
ABSTRACT: Two Aeromonas salmonicida-specific polymerase chain reaction (PCR) tests and 1 A. salmonicida subsp. salmonicida-specific PCR test were used to screen salmonid populations that were either overtly or covertly infected with
A. salmonicida subsp. salmonicida. It was demonstrated that these PCR assays could be used to replace the biochemical testing currently employed to confirm the identity of A. salmonicida isolates cultured from infected fish. The AP
and PAAS PCR assays were also capable of direct detection of A. salmonicida in overtly infected fish, with mucus, gill and kidney samples most likely to yield a positive result. Culture was a more reliable method for the direct detection of A.
salmonicida in covertly infected salmonids than was the direct PCR testing of tissue samples, with the AP and PAAS PCRs having a lower detection limit (LDL) of approximately 4 ¥ 105 colony-forming units (CFU)
g-1 sample.
KEY WORDS: Aeromonas salmonicida · Polymerase chain reaction · Detection · Identification · Validation · Naturally infected fish · Covert infections
Full text in pdf format
Published in DAO Vol.
49, No. 2
(2002) on May 10
Print ISSN: 0177-5103; Online ISSN: 1616-1580.
Copyright © Inter-Research, Oldendorf/Luhe, 2002
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