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DAO 49:123-128 (2002)
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Abstract
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Detection of infectious salmon anemia virus in sea water by nested RT-PCR
Trond Løvdal1,*, Øivind Enger2
1Department of Microbiology, University of Bergen, Jahnebakken 5, PO Box 7800, 5020 Bergen, Norway
2 Forinnova AS, Thormøhlensgate 55, 5008 Bergen, Norway
*E-mail: trond.lovdal@im.uib.no
ABSTRACT: A method to detect low levels of infectious salmon anemia virus (ISAV) in environmental samples has been developed. The method is based on concentrating the viruses by tangential flow filtration and ultracentrifugation prior to amplification of
the extracted viral RNA by nested reverse transcription polymerase chain reaction (RT-PCR). In the current investigation, seawater samples from salmon holdings were used for ISAV identification. ISAV was detected in seawater samples from a salmon holding
site and from a vessel transporting salmon in 2 consecutive trials of the methodology. When known concentrations of ISAV were added to 2 l of sea water, 5.5 viruses ml-1 (corresponding to a tissue culture titer of TCID50 1.6 ¥ 10-2 ml-1) were detected using this method.
KEY WORDS: Infectious salmon anemia virus · Nested RT-PCR · Virus concentration · Salmo salar
Full text in pdf format
Published in DAO Vol.
49, No. 2
(2002) on May 10
Print ISSN: 0177-5103; Online ISSN: 1616-1580.
Copyright © Inter-Research, Oldendorf/Luhe, 2002
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