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DAO 47:33-38 (2001)
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Abstract
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Sea bream Sparus aurata, an asymptomatic contagious fish host for nodavirus
J. Castric1,*, R. Thiéry1, J. Jeffroy1, P. de Kinkelin2, J. C. Raymond3
1Afssa Site de Brest, BP 70, 29280 Plouzané, France
2INRA, Unité de Virologie et d'Immunologie Moléculaires, Laboratoire de Pathologie Infectieuse et Immunité des Poissons, 78352 Jouy-en-Josas cedex, France
3SAVU-CNPMEM, Les Amandiers, Rue Berthe Morisot, 34430 St-Jean de Védas, France
*E-mail: j.castric@brest.afssa.fr
ABSTRACT: During an epidemiological survey of viral encephalopathy and retinopathy (VER) in diseased sea bass Dicentrarchus labrax, a nodavirus isolate was recovered from net pen-reared sea bream Sparus aurata harboured in the same farming
premises. After the virus was isolated and identified by immunofluorescence on SSN-1 cells, sequence analysis with a PCR product from the T4 region of the capsid protein gene indicated that the virus shared 100% identity with a pathogenic virus strain
isolated from sea bass. Infection trials demonstrated the pathogenicity of the sea bream virus isolate for juvenile sea bass whereas sea bream infected with the same virus isolate remained asymptomatic even following intramuscular injection of virus.
Nevertheless, the sea bream appeared to be a potential carrier of nodavirus, as juvenile sea bass became infected when maintained in a tank containing experimentally contaminated sea bream.
KEY WORDS: Nodavirus · Sea bream · Sea bass · Polymerase chain reaction · Sequencing · Virulence
Full text in pdf format
Published in DAO Vol.
47, No. 1
(2001) on October 29
Print ISSN: 0177-5103; Online ISSN: 1616-1580.
Copyright © Inter-Research, Oldendorf/Luhe, 2001
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