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DAO 46:115-121 (2001)
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Abstract
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Identity of Naegleria strains isolated from organs of freshwater fishes
I. Dyková1,2,*, I. Kyselová1,2, H. Pecková1, M. Oborník1,2, J. Lukes1,2
1Institute of Parasitology, Academy of Sciences of the Czech Republic, and
2Faculty of Biological Sciences, University of South Bohemia, Branisovská 31,
370 05 Ceské Budejovice, Czech Republic
*E-mail: iva@paru.cas.cz
ABSTRACT: Eighteen Naegleria strains were isolated from organs of freshwater fishes belonging to 5 species. Morphometric study allowed the separation of the Naegleria strains from the non-vahlkampfiid amoeboflagellates, but was inadequate
for species determination. Six strains, representatives of groups that had a slightly different cyst size, were selected and corresponding derived clones were subjected to sequence analysis and riboprinting restriction fragment length polymorphism
(RFLP)-PCR analysis of the small subunit (SSU) rRNA genes. One strain isolated from the brain of a fish with systemic infection was characterised by an intronless 2 kb long SSU rRNA gene and was identified as N. australiensis. Another 5 strains had
a 1.3 kb long group I intron in their SSU rRNA gene and, based on the SSU rRNA sequences and riboprints, RFLP-PCR patterns appeared in phylogenetic trees to be closely related to Naegleria clarki.
KEY WORDS: Naegleria spp. · Fish strains · Molecular taxonomy · SSU rRNA
Full text in pdf format
Published in DAO Vol.
46, No. 2
(2001) on September 12
Print ISSN: 0177-5103; Online ISSN: 1616-1580.
Copyright © Inter-Research, Oldendorf/Luhe, 2001
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