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DAO 35:149-153 (1999)
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Abstract
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Virus susceptibility of the fish cell line SAF-1 derived from gilt-head seabream
S. I. Perez-Prieto1, S. Rodriguez-Saint-Jean1, E. Garcia-Rosado2, D. Castro2, M. C. Alvarez3, J. J. Borrego2,*
1Centro de Investigaciones Biológicas, C.S.I.C., Velázquez, 144, E-28006 Madrid, Spain
2Departamento de Microbiología and 3Departamento de Biología Celular y Genética, Facultad de Ciencias, Universidad de Málaga, E-29071 Málaga, Spain
*Addressee for correspondence. E-mail: jjborrego@ccuma.uma.es
ABSTRACT: The recently reported SAF-1 cell line from fins of gilt-head seabream was evaluated for susceptibility to lymphocystis disease virus (LDV) and to several salmonid fish viruses, such as infectious haematopoietic necrosis virus (IHNV), viral
haemorrhagic septicemia virus (VHSV) and several strains of infectious pancreatic necrosis virus (IPNV). LDV, VHSV and IHNV replicated well in the cultured fin cells as demonstrated by cell lysis and increases in viral titer. The potential use of this
cell line to detect viruses from fish marine species is discussed.
KEY WORDS: Fish viruses · Lymphocystis · Fish cell lines · SAF-1 · Gilt-head seabream
Published in DAO Vol.
35, No. 2
(1999) on January 29
ISSN: 0177-5103.
Copyright © Inter-Research, Oldendorf/Luhe, 1999
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