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DAO 35:101-105 (1999)
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Abstract
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Different prevalences of Renibacterium salmoninarum detected by ELISA in Alaskan chinook salmon Oncorhynchus tshawytscha spawned from freshwater and seawater
T. R. Meyers1,*, F. Thrower2, S. Short1, K. Lipson1, J. Joyce2, C. Farrington3, S. Doherty4
1Alaska Department of Fish and Game, Commercial Fisheries Division, PO Box 25526, Juneau, Alaska 99802-5526, USA
2National Marine Fisheries Service, Auke Bay Laboratory, 11305 Glacier Highway, Juneau, Alaska 99801, USA
3Alaska Department of Fish and Game, Commercial Fisheries Division, 802 3rd Street, PO Box 240020, Douglas, Alaska 99824-0020, USA
4Southern Southeast Regional Aquaculture Association, 2721 Tongass Avenue, Ketchikan, Alaska 99901, USA
*E-mail: fishpath%fishgame@state.ak.us

ABSTRACT: Soluble antigen of Renibacterium salmoninarum (Rs) was detected by a polyclonal enzyme-linked immunosorbent assay (ELISA) at significantly higher prevalences in adult chinook salmon Oncorhynchus tshawytscha that matured in
freshwater than in the same cohort of fish spawned after maturation in seawater. The cumulative results were consistent during 4 yr of comparison at the Little Port Walter Hatchery on Baranof Island, Alaska, USA. Possible causes for this difference are
discussed. Maturation of chinook salmon broodstock in seawater has become a practical strategy at this hatchery to reduce the prevalence of Rs-positive parent fish and the numbers of culled eggs.
KEY WORDS: Renibacterium · ELISA · Chinook

Published in DAO Vol.
35, No. 2
(1999) on January 29
ISSN: 0177-5103.
Copyright © Inter-Research, Oldendorf/Luhe, 1999
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