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DAO 32:87-90 (1998)
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Abstract
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Rapid diagnosis of red sea bream iridovirus infection using the polymerase chain reaction
Syunichirou Oshima1, Jun-ichiro Hata1, Noritaka Hirasawa2, Taro Ohtaka2, Ikuo Hirono3, Takashi Aoki3, Shinya Yamashita1,*
1Central Research Laboratory, Nippon Suisan Kaisha Ltd, 559-6, Kitanomachi, Hachioji, Tokyo 192, Japan
2Ohita Marine Center, Nippon Suisan Kaisha Ltd, Tsurumicho, Minamiamabegun, Ohita 508-8, Japan
3Department of
Aquatic Biosciences, Tokyo University of Fisheries, Konan, Minato, Tokyo 108, Japan
*Addressee for correspondence. E-mail: kye00332@niftyserve.or.jp
ABSTRACT: A simple and sensitive polymerase chain reaction (PCR) based assay is described for detection of the red sea bream iridovirus (RSIV) in infected fish. The assay involves amplification of a portion of the ribonucleotide reductase small subunit (RNRS) gene
of the virus from DNA isolated from the spleen. The system was tested on red sea bream following an experimental infection. In our infection model, disease signs first became apparent 5 to 6 d post-infection, and mortality commenced at Day 6 and reached
90% by Day 9. No amplified product was detected from fish at 1 or 2 d post-infection, but 3 of 5 fish tested positive at Day 3, and all fish tested positive at Days 5 and 8. Thus, infection could be detected prior to the appearance of overt symptoms. This
PCR method should be of considerable value for aquaculture to detect RSIV infection.
KEY WORDS: Red sea bream · PCR · RSIV · Iridovirus · Infection
Published in DAO Vol.
32, No. 2
(1998) on March 5
ISSN: 0177-5103.
Copyright © Inter-Research, Oldendorf/Luhe, 1998
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