ABSTRACT: In Taiwan, 3 viral isolates were made from a disease outbreak in rainbow trout Oncorhynchus mykiss cultured in ponds during the period from November 1994 to February 1995. Polymerase chain reaction (PCR) was used for identification of the isolates. The results revealed that a specific 252 bp viral nucleotide was amplified by using infectious hematopoietic necrosis virus (IHNV) specific primers. At the same time, several IHNV monoclonal antibodies (MAbs), and an IHNV specific rabbit polyclonal antibody were used to confirm the results of the PCR assay. The results of the above assays indicated that all isolates were IHNV.

KEY WORDS: IHNV · PCR · MAb · Taiwan · Fish · Virus

Published in DAO Vol. 26, No. 3 (1996) on September 12
ISSN: 0177-5103. Copyright © Inter-Research, Oldendorf/Luhe, 1996