IR Home
MEPS
Home
Editors
Forthcoming
Information
Subscribe
Journals
Home
MEPS
AME
CR
DAO
ESEP
ESR
Search
Subscribe
Book Series
EE Books
Top Books
ESEP Books
Order
EEIU Brochures
(pdf format)
Discussion Forums
Home
Research
IR Research
Institutions
International Ecology Institute
Eco-Ethics International Union
Foundation
Otto Kinne Foundation
 | |
MEPS 273:89-96 (2004)
|
Abstract
|
Coral-associated Archaea
Linda Wegley1, Yanan Yu1, Mya Breitbart1, Veronica Casas1, David I. Kline1,2,3, Forest Rohwer1,4,*
1Department of Biology, LS316, San Diego State University, 5500 Campanile Dr, San Diego, California 92182-4614, USA
2University of California, San Diego, Scripps Institution of Oceanography, La Jolla, California 92093-0202, USA
3Smithsonian Tropical Research Institute, Apartado 2072, Balboa, Panama
4Center for Microbial Sciences, San Diego State University, 5500 Campanile Dr, San Diego, California 92182, USA
*Corresponding author. Email: forest@sunstroke.sdsu.edu
ABSTRACT: The coral holobiont includes the coral, zooxanthellae, fungi, endolithic algae, and >30 species of Bacteria. Using culture-independent techniques, we now show that Archaea are also abundant and widespread on corals. Sequence analyses of
Archaea on 3 species of Caribbean corals revealed that coral-associated Archaea are novel, diverse, and include representatives from both the Crenarchaeota and Euryarchaeota. Unlike zooxanthellae and Bacteria, the Archaea do not appear to form
species-specific associations with reef-building corals. Fluorescent in situ hybridizations with peptide nucleic acid (PNA) probes showed that Archaea were present at >107 cells cm-2 on Porites astreoides, comprising
nearly half of the prokaryotic community. This study and one by Kellogg (Mar Ecol Prog Ser 273:81-88) show that Archaea are abundant, diverse, and potentially important components of the coral holobiont.
KEY WORDS: Coral · Archaea · 16S rDNA · Fluorescent in situ hybridization · FISH · Peptide nucleic acid probe · PNA
Full text in pdf format
Published in MEPS Vol.
273
(2004) on June 8
Print ISSN: 0171-8630; Online ISSN: 1616-1599.
Copyright © Inter-Research, Oldendorf/Luhe, 2004
|
