IR Home
MEPS
Home
Editors
Forthcoming
Information
Subscribe
Journals
Home
MEPS
AME
CR
DAO
ESEP
Search
Subscribe
Book Series
EE Books
Top Books
ESEP Books
Order
EEIU Brochures
(pdf format)
Discussion Forums
Home
Research
Endangered Species Programs
Institutions
International Ecology Institute
Eco-Ethics International Union
Foundation
Otto Kinne Foundation
 | |
MEPS 262:229-240 (2003)
|
Abstract
|
Relationship of RNA/DNA ratio and temperature to growth in larvae of Atlantic cod Gadus morhua
Elaine M. Caldarone1,*, Jeanne M. St. Onge-Burns1, Lawrence J. Buckley2
1National Oceanic and Atmospheric Administration National Marine Fisheries Service, Northeast Fisheries Science Center, Narragansett Laboratory, 28 Tarzwell Drive, Narragansett, Rhode Island 02882-1199, USA
2URI/NOAA CMER Program, Graduate School of Oceanography, University of Rhode Island, Narragansett, Rhode Island 02882, USA
*Email: elaine.caldarone@noaa.gov
ABSTRACT: The RNA, DNA, and protein content of larval cod Gadus morhua reared in the laboratory at 3 temperatures and 3 densities of prey was measured. The data were used to define a quantitative relationship between RNA/DNA ratio (R/D), water
temperature (T), and protein-specific growth rate (SGR, % d-1). The nucleic acid content of each larva was determined with both a 2-dye flow-injection fluorometric assay (FIA) and a 1-dye/1-enzyme fluorometric microplate assay (MFA) in
order to calibrate each methodology. The resulting equations were: SGR = 3.65 R/D + 1.02 T - 13.05 for FIA, and SGR = 4.03 R/D + 0.88 T - 11.16 for MFA. Measured growth rates ranged from negative (-8% d-1) to 20% d-1. Water
temperature and larval R/D explained 37 to 39% of the variability in the observed growth rate. The models are applicable over temperatures ranging from 2.5 to 9.5°C and can be used to determine short-term growth rates of cod larvae collected from both the
laboratory and field.
KEY WORDS: RNA/DNA ratio · Growth · Cod · Larvae · Temperature effects
Full text in pdf format
Published in MEPS Vol.
262
(2003) on November 7
Print ISSN: 0171-8630; Online ISSN: 1616-1599.
Copyright © Inter-Research, Oldendorf/Luhe, 2003
|
