Inter-Research
Aquatic Microbial Ecology

ABSTRACT: Seasonal and diurnal variation of denitrifying activity was determined in a microphyte-covered lowland stream sediment using an isotope pairing technique based on the stable ¹⁵N-isotope. By this approach it was possible to divide total denitrification (D_tot) into denitrification based on NO₃^- diffusing from the overlying water (D_w) and coupled nitrification-denitrification within the sediment (D_n). D_tot exhibited seasonal variations between 0 and 17 mmol m^-2 d^-1 with a pronounced minimum during winter. D_w showed the same seasonal pattern as D_tot and accounted for 75 to 90% of the total denitrification activity. In contrast to the pattern previously found in estuarine systems, a minimum in D_w activity was found during the winter period when the NO₃^- concentration in the water was highest. This was apparently due to a dramatic increase in water discharge during the winter period increasing the erosion of the stream bed, thereby reducing the carbon availability for the denitrifiers and probably also their numbers. Temporary sediment N accumulation was estimated as the difference between rates of total net dissolved inorganic nitrogen uptake (NH₄⁺, NO₃^-) and total denitrification (D_n +D_w). The rate of temporary sediment N accumulation varied seasonally between 0 and 13 mmol N m^-2 d^-1 with a pronounced maximum during spring (April-May) coincident with the microphytobentic spring bloom. Most of the N load to the stream occurred during the winter and as a consequence the retention capacity of the system was low on an annual basis. Denitrification and accumulation in the stream were both found to account for less than 1% of the N transported on an annual basis, while approximately 60% was removed by denitrification and sediment accumulation during the summer period (May-August).

KEY WORDS: Denitrification · N accumulation · N load

Published in AME Vol. 12, No. 3 (1997) on May 29
Print ISSN: 0948-3055; Online ISSN: 1616-1564. Copyright © Inter-Research, Oldendorf/Luhe, 1997