Inter-Research
Aquatic Microbial Ecology

ABSTRACT: A simple and rapid method for microscopic quantification of an abundant class of labile pico- and nano-sized detrital particles (0.2 to 20 um), using the fluorochrome stain DAPI, is described. Using an appropriate UV-filter set, examination of DAPI-stained samples revealed not only blue (DNA-containing) but also yellow particulate matter. We showed that DAPI yellow fluorescing particles resembled detritus made from plankton tow material or copepod faecal pellets and were almost exclusively organic, enzyme-degradable matter. Quantification of the total surface area of these particles in the Ligurian Sea (NW Mediterranean) revealed stocks averaging 80 mm² l^-1 in surface layer waters. The possible origin and fate of these pico- and nano-sized DAPI Yellow Particles (DYP) are discussed.

KEY WORDS: Detrital particles . Detritus . Staining . DAPI Yellow Particles (DYP)

Published in AME Vol. 9, No. 3 (1995) on December 21
Print ISSN: 0948-3055; Online ISSN: 1616-1564. Copyright © Inter-Research, Oldendorf/Luhe, 1995